Sheffield Wednesday exited the Johnstone's Paint Trophy at Bradford on Tuesday, losing 3-1 on penalties after a goalless 90 minutes.
The Bantams had by far the better chances, striking the woodwork once, seeing another effort cleared from the line and also bringing the best out of Richard O'Donnell.
But it was the home side who advanced through to the next round on penalties, with Clinton Morrison, Liam Palmer and Chris Sedgwick missing from the spot.
Wednesday included academy defender Ayo Obileye, who at 16-years-old became one of the youngest players ever to represent the Owls at senior level.
The first talking point was substitute goalkeeper O'Donnell coming on for Nicky Weaver for Wednesday with just over a minute on the clock.
And O'Donnell would almost certainly have been tested on the 15-minute mark when Mark Stewart found space in the penalty area but Mark Reynolds poked wide for a corner just as the striker was about to pull the trigger.
The Owls made two more changes on 19 minutes, with Jose Semedo and David Prutton making way for Giles Coke and Cecil Nyoni.
The visitors' first opening came midway through the half when Morrison span in the area and shot but was denied by a timely block by Robbie Threlfall.
The same pair were at the heart of the action a few minutes later, with the Bradford man coming out on top again to deflect Morrison's angled drive.
Overall, Wednesday shaded possession through the opening period, although clear cut opportunities were at a premium for both sides - until right on the whistle when James Hanson headed wide for the hosts with the goal at his mercy.
Bradford then had another golden chance immediately after the restart. Oboleye's header back to O'Donnell was short and Jack Compton nipped in but his header bounced the wrong side of the post.
O'Donnell saved smartly from Mark Stewart as the Bantams upped the ante, while the Owls responded with James O'Connor rattling in a low effort that fizzed wide.
Play promptly reverted to the other end, though, and Luke Oliver came close to breaking the deadlock with a glancing header but Nyoni was well-placed on the line to clear.
Palmer got to the by-line for the Owls as the clock ticked down and drove a delicious cross in that evaded a packed danger zone.
The Bantams went close once more seven minutes from time when Ritchie Jones launched a thundering volley from 15 yards that forced an excellent reflex save from O'Donnell.
They went closer still right on the whistle as Threlfall delivered an accurate free kick into the box that the unlucky Oliver headed onto the crossbar.
So penalties it was and the Owls got off to the worst possible start with Morrison blazing over the crossbar and Palmer also missing Wednesday's next spot kick.
Following Coke's successful penalty, Sedgwick had to score to keep Wednesday's hopes alive but Bradford keeper Jansson made a superb save from the spot to knock the Owls out of the JPT in round one.
Bradford: Jansson, Flynn, Branston, Oliver, Stewart (Hannah 62), Jones, Hanson, Compton (O'Brien 85), Mitchell (Bryan 72), Moore, Threlfall
Unused substitutes: McLaughlin, Hunt
Owls: Weaver (O'Donnell 2), Palmer, Obileye, Beevers, Reynolds, Semedo (Coke 18), Prutton (Nyoni 18), Sedgwick, O'Connor, Morrison, Uchechi
Unused substitutes: Lines, Madine