Owls defender Anthony Gardner has said the squad will quickly pick themselves up and get straight down to the business of preparing for the season finale.
Wednesday host Middlesbrough at Hillsborough on Saturday and need victory to guarantee second tier survival.
Following defeat against Peterborough, the Owls head into the last day of the season with destiny still in their own hands - one point clear of the drop.
“The key thing is it’s down to us what will happen,” Gardner said.
“The boys all know what is needed and we’ll give it everything we have next week.
“We have to be positive, we have a home game against Middlesbrough on the final day and we need to get a result, it’s as simple as that.
“It’s the last game of the season, it’s in our hands but we need the points to guarantee that we stay in this division.”
Boro have endured a stuttering end to the term with just one win in ten and seven defeats from that spell but Gardner is expecting nothing less than a difficult afternoon in S6.
He added: “There’s never a good time to play any team. Middlesbrough are safe and can come here and play without pressure.
“They will come to Hillsborough and try to do a professional job and we have to match that and use our strengths to win the game.
“It’s a huge game and we’ll be aiming to go out there and get the three points that are up for grabs. Of course there is added pressure but that’s the nature of this game.”