Wednesday duo Barry Bannan and Steven Fletcher both featured on Tuesday evening as Scotland suffered their first World Cup qualification defeat.
The Scots went down 3-0 to Slovakia in Trnava, leaving them fourth in Group F behind England, Lithuania and Slovenia.
Bannan retained his place in the centre of midfield and has now started all three of his country’s qualifying fixtures.
Meanwhile, Fletcher was recalled to the starting XI. The Owls hitman was an unused substitute in the 1-1 draw with Lithuania and before that, scored from the bench in the opening victory over Malta.
There was controversy surrounding the first goal in Trnava, as Fletcher was brought down after making a well-timed interception but his strong case for a free kick was waved away.
From there, the hosts broke upfield with devastating effect as Robert Mak smashed home from six yards.
Robert Snodgrass then went close after collecting a neat feed from Bannan but was denied by a smart save from Matúš Kozácik.
Further goals from Mak and Adam Nemec widened the scoreline after the break, leaving the Scots with work to do in the group.
Next up for Bannan, Fletcher and co. in their World Cup 2018 qualification bid is the clash with England at Wembley on 11 November.