The Owls Under-14s and Under-16s will compete in the Youdan Trophy which begins on Monday.
Between 31 July and 4 August, more than 800 young players from some of the world's most celebrated clubs will converge on Sheffield to contest the sport's oldest trophy.
The Under-14s’ event will be staged at the Warminster Road facility of the University of Sheffield.
In the group stages, Wednesday - who made it to the final in 2016 - will face Birmingham City (12pm) on Monday 31 July.
The following day, a clash with Kawasaki Frontale will take place at midday.
Then to round off the group stage, the Owls will take on Charlton (12pm) and Houston Dynamo (5.00pm).
For the Under-16s, who will compete at the Sheffield Hallam University Sports Park, the Owls begin on Monday with a tough opening clash against Newcastle (2.30pm).
On Tuesday 1 August, Wednesday have an early start against Accrington (9.30am) and face Crystal Palace later in the day (2.30pm).
Rounding off the group phase, the Under-16s have a lunchtime encounter with Swiss side FC Basel.
This year the Youdan Trophy final will be officiated by former Premier League referee Mark Clattenburg.
Keith Hackett, director of tournament organisers You-Are-The-Ref.com said: “Mark will be refereeing his first game in this country since moving on to pastures new – what a great thrill that will be for the young players.
“The Youdan Trophy continues to grow and grow and is fast developing into the biggest and most prestigious tournament of its kind in the whole of Europe.
“We are confident that in the coming years it will be established as a globally recognizable Sheffield brand.”