The Owls and Barnsley are putting local rivalries aside to join forces and back United Nations Anti-Racism day this weekend.
The Championship fixture at Oakwell will see both teams take the knee ahead of kick-off as football continues to vehemently oppose any form of racism or discrimination.
Stand Up To Racism groups in Sheffield and Barnsley initiated the matchday move to mark the international day of activities that will be visible across the UK.
Phil Turner of Sheffield Stand Up To Racism said: "We believe teams can be rivals on the pitch but united against racism.
"Racism has no place in football or wider society and we believe that more has to be done to kick racism out of our game once and for all.
"Great work has been done by Sheffield-based Football Unites, Racism Divides and we aim to build on that tradition."
Owls boss Darren Moore added: “We’re all joined, we’re all unified against any kind of racism which we are trying to eradicate and stamp out.
“It’s a really positive step that both clubs will be right behind this campaign on Saturday as it’s so important we all continue to send the message that racism and discrimination is unacceptable in all walks of life.”